Utilization
Fraki is helpful in two fields of proteomic researches.
Protein sequence research: The measured data are compared with the data from the theoretical digestion.
One can chose between 50, 100, 150 ppm mass tolerances. The program selects the peptides with masses in
the chosen mass tolerance range according to the theoretical digestion data. The masses from the
theoretical digestion, the sequence and the location of the peptides given in a reference file, are
ordered to the measured mass data. The sequence of the identified peptide is fitted to the sequence
of the protein (the unique parts are shown with red colour) and the coverage is given in percentage.
Posttranslational modification research: The mass generated from the digestion of the unmodified protein
(taking account the possible mass changes arising from the posttranslational modifications) is compared
with the measured mass data in the mass tolerance range, 50, 100, 150 ppm. The generated mass, the sequence
and the location of the unmodified peptide are ordered to the identified masses. This allows the
definition of the modification sites on the protein (peptide).
Specification
- Capable of computing with up to 1000 units of input data and reference data
- Formatted evaluation into Excel sheets
- Precision of 8 decimals
System requirements
- Windows XP operating system with at least Service Pack 1
- Microsoft .NET Framework
- Microsoft Office 2003 Excel